Please
feel free to email with
any questions. We'll try our best to respond.
Xenopus
Egg Extractology
Crude
Extract Prep for In Vitro Spindle Assembly.
High
Speed Supe Extract Prep
Crude
Extract Immunodepletion
HSS
Extract Immunodepletion
Microtubules
Tubulin
Basics
Large
Scale Tubulin Preparation
Recycling
Tubulin
Labeling
Tubulin and Quantifying Labeling Stoichiometry
Tubulin
Polymerization with GTP/GMPCPP/Taxol
Preparation
of Segmented and Polarity Marked Microtubules
Microtubule
Spindowns for Visual Analysis
Flow
Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and
VE-DIC
Negative
Stain Electron Microscopy of Microtubules
Centrosome
Prep
Microtubule
Pelleting
Chromosomes
& Kinetochores
CHO
Chromosome Prep
In
Vitro Chromatin and Chromosome Assembly
Pelleting
Assembled Chromatin for Microscopy
Antibodies
Preparation
Of Peptide-KLH Conjugates For Immunization
Testing
Rabbit Bleeds By Elisa
Coupling
Peptides To Resin For Affinity Purification
Purification
Of Anti Peptide Antibody
General
Techniques
Fixation
and Immunofluorescence of the Cytoskeleton
Microinjection
of Single Cells in Culture
In-Gel
Tryptic Digest for Mass Spec