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Xenopus Egg Extractology

Crude Extract Prep for In Vitro Spindle Assembly.

High Speed Supe Extract Prep

Crude Extract Immunodepletion

HSS Extract Immunodepletion

Microtubules

Tubulin Basics

Large Scale Tubulin Preparation

Recycling Tubulin

Labeling Tubulin and Quantifying Labeling Stoichiometry

Tubulin Polymerization with GTP/GMPCPP/Taxol

Preparation of Segmented and Polarity Marked Microtubules

Microtubule Spindowns for Visual Analysis

Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC

Negative Stain Electron Microscopy of Microtubules

Centrosome Prep

Microtubule Pelleting

Chromosomes & Kinetochores

CHO Chromosome Prep

In Vitro Chromatin and Chromosome Assembly

Pelleting Assembled Chromatin for Microscopy

Antibodies

Preparation Of Peptide-KLH Conjugates For Immunization

Testing Rabbit Bleeds By Elisa

Coupling Peptides To Resin For Affinity Purification

Purification Of Anti Peptide Antibody

General Techniques

Fixation and Immunofluorescence of the Cytoskeleton

Microinjection of Single Cells in Culture

In-Gel Tryptic Digest for Mass Spec