Jue Shi I'm interested in systematic and quantitative studies of long-term anti-cancer drug response using high throughput microscopy. I'll be looking at cell phenotypic profiles relevant to both mitosis and cancer by screening drugs on different cell-lines with distinct genetic variations, in an attempt to understand how drugs or combinations of drugs would impact the multiple pathways involved in mitosis and cancer.

Email: jue_shi * hms.harvard.edu

Publications Huang HC, Shi J, Orth JD, Mitchison TJ. Evidence that Mitotic Exit Is a Better Cancer Therapeutic Target Than Spindle Assembly. Cancer Cell. 2009 Oct 6;16(4):347-58. PubMed Shi J, Dertouzos J, Gafni A, Steel D. Application of single-molecule spectroscopy in studying enzyme kinetics and mechanism. Methods Enzymol. 2008;450:129-57. PubMed Orth JD, Tang Y, Shi J, Loy CT, Amendt C, Wilm C, Zenke FT, Mitchison TJ. Quantitative live imaging of cancer and normal cells treated with Kinesin-5 inhibitors indicates significant differences in phenotypic responses and cell fate. Mol Cancer Ther. 2008 Nov;7(11):3480-9. Epub 2008 Oct 30. PubMed Shi J, Orth JD, Mitchison T. Cell type variation in responses to antimitotic drugs that target microtubules and kinesin-5. Cancer Res. 2008 May 1;68(9):3269-76. PubMed Shi J, Gafni A, Steel D. Simulated data sets for single molecule kinetics: some limitations and complications of data analysis. Eur Biophys J. 2006 May 5; [Epub ahead of print] PubMed Shi J, Dertouzos J, Gafni A, Steel D, Palfey BA. Single-molecule kinetics reveals signatures of half-sites reactivity in dihydroorotate dehydrogenase A catalysis. Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):5775-80. Epub 2006 Apr 3. PubMed Shi J, Palfey BA, Dertouzos J, Jensen KF, Gafni A, Steel D. Multiple states of the Tyr318Leu mutant of dihydroorotate dehydrogenase revealed by single-molecule kinetics. J Am Chem Soc. 2004 Jun 9;126(22):6914-22. PubMed