James Orth I'm interested in the function of the mitotic spindle during tumor cell division. Multipolar mitosis (ie tripolar metaphase rather than bipolar) as a function of centrosome amplification, identification of novel proteins involved, and the role of Eg5 in this process is being explored.

Email: james_orth * hms.harvard.edu

Publications Tsui M, Xie T, Orth JD, Carpenter AE, Rudnicki S, Kim S, Shamu CE, Mitchison TJ. An intermittent live cell imaging screen for siRNA enhancers and suppressors of a kinesin-5 inhibitor. PLoS One. 2009 Oct 5;4(10):e7339. PubMed Huang HC, Shi J, Orth JD, Mitchison TJ. Evidence that Mitotic Exit Is a Better Cancer Therapeutic Target Than Spindle Assembly. Cancer Cell. 2009 Oct 6;16(4):347-58. PubMed Orth JD, Tang Y, Shi J, Loy CT, Amendt C, Wilm C, Zenke FT, Mitchison TJ. Quantitative live imaging of cancer and normal cells treated with Kinesin-5 inhibitors indicates significant differences in phenotypic responses and cell fate. Mol Cancer Ther. 2008 Nov;7(11):3480-9. Epub 2008 Oct 30. PubMed Shi J, Orth JD, Mitchison T. Cell type variation in responses to antimitotic drugs that target microtubules and kinesin-5. Cancer Res. 2008 May 1;68(9):3269-76. PubMed Orth JD, McNiven MA. Get off my back! Rapid receptor internalization through circular dorsal ruffles. Cancer Res. 2006 Dec 1;66(23):11094-6. PubMed Orth JD, Krueger EW, Weller SG, McNiven MA. A novel endocytic mechanism of epidermal growth factor receptor sequestration and internalization. Cancer Res. 2006 Apr 1;66(7):3603-10. PubMed Cao H, Weller S, Orth JD, Chen J, Huang B, Chen JL, Stamnes M, McNiven MA. Comment in: Nat Cell Biol. 2005 May;7(5):448-9. Nat Cell Biol. 2005 Apr 10; [Epub ahead of print] PubMed Yao Q, Chen J, Cao H, Orth JD, Michael McCaffery J, Stan RV, McNiven MA. Caveolin-1 interacts directly with dynamin-2. J Mol Biol. 2005 Apr 29;348(2):491-501. PubMed Buccione R, Orth JD, McNiven MA. Foot and mouth: podosomes, invadopodia and circular dorsal ruffles. Nat Rev Mol Cell Biol. 2004 Aug;5(8):647-57. PubMed Chen XM, Huang BQ, Splinter PL, Orth JD, Billadeau DD, McNiven MA, LaRusso NF. Cdc42 and the actin-related protein/neural Wiskott-Aldrich syndrome protein network mediate cellular invasion by Cryptosporidium parvum. Infect Immun. 2004 May;72(5):3011-21. PubMed Krueger EW, Orth JD, Cao H, McNiven MA. A dynamin-cortactin-Arp2/3 complex mediates actin reorganization in growth factor-stimulated cells. Mol Biol Cell. 2003 Mar;14(3):1085-96. PubMed Cao H, Orth JD, Chen J, Weller SG, Heuser JE, McNiven MA. Cortactin is a component of clathrin-coated pits and participates in receptor-mediated endocytosis. Mol Cell Biol. 2003 Mar;23(6):2162-70. PubMed Orth JD, McNiven MA. Dynamin at the actin-membrane interface. Curr Opin Cell Biol. 2003 Feb;15(1):31-9. PubMed Orth JD, Krueger EW, Cao H, McNiven MA. The large GTPase dynamin regulates actin comet formation and movement in living cells. Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):167-72. PubMed Hart PE, Poynter GM, Whitehead CM, Orth JD, Glantz JN, Busby RC, Barrett SL, Salisbury JL. Characterization of the X-linked murine centrin Cetn2 gene. Gene. 2001 Feb 21;264(2):205-13. PubMed McNiven MA, Kim L, Krueger EW, Orth JD, Cao H, Wong TW. Regulated interactions between dynamin and the actin-binding protein cortactin modulate cell shape. J Cell Biol. 2000 Oct 2;151(1):187-98. PubMed Hart PE, Glantz JN, Orth JD, Poynter GM, Salisbury JL. Testis-specific murine centrin, Cetn1: genomic characterization and evidence for retroposition of a gene encoding a centrosome protein. Genomics. 1999 Sep 1;60(2):111-20. PubMed